| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.186900 |
| Chromosome: | chromosome 11 |
| Location: | 1587358 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467759 | PIC1,PIC1-2 | (1 of 2) PTHR34548:SF2 - PROTEIN TIC 21, CHLOROPLASTIC; Similar to AtTic21/PIC1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGATTCCCTGTAAAGCTGTAAAGATGGTCT |
| Internal bar code: | TCTTGTGGGAGCGCGCGGACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 412 |
| LEAP-Seq percent confirming: | 99.6216 |
| LEAP-Seq n confirming: | 6318 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAAACAAGCAGGCCGCTAC |
| Suggested primer 2: | AGACCACAAGGTCTGGGTTG |