| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.186912 |
| Chromosome: | chromosome 6 |
| Location: | 6741607 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294550 | (1 of 1) IPR000104//IPR008537 - Antifreeze protein, type I // Protein of unknown function DUF819 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCTACCGCTGCCTACCTGGGCTAGTGCG |
| Internal bar code: | TCGCATACGGGGAGGTCGAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 82.0852 |
| LEAP-Seq n confirming: | 559 |
| LEAP-Seq n nonconfirming: | 122 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGACGTCAATGACCAATCG |
| Suggested primer 2: | GTACCATGTGCGATGTTTCG |