| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.186913 |
| Chromosome: | chromosome 7 |
| Location: | 341165 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314550 | TRM8,TMG9 | (1 of 1) PTHR23417//PTHR23417:SF16 - 3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE // TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE; tRNA (guanine-N7)-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCCTCGCGGCAATGCAGTACATGCCGA |
| Internal bar code: | CTTGTGACAAGCGCAGGGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 180 |
| LEAP-Seq percent confirming: | 99.8652 |
| LEAP-Seq n confirming: | 2222 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTGTATGCCATGGAACG |
| Suggested primer 2: | TTGTGTTGGTGAGTGGCATT |