Insertion junction: LMJ.RY0402.186915_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.186915
Chromosome:chromosome 9
Location:1108250
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g400312 (1 of 5) IPR001680//IPR011047//IPR017986 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // WD40-repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCGAACATCAAGTGTTTGCTCAGCACAT
Internal bar code:TCGCGTGTCTTAGTGCACAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:344
LEAP-Seq percent confirming:99.7268
LEAP-Seq n confirming:730
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTAAGTCCAGCCTTTGCTGC
Suggested primer 2:CCGGTGTGAAGCATACCTTT