| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.186940 |
| Chromosome: | chromosome 13 |
| Location: | 1346488 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571500 | CST2 | Chlamydomonas-specific membrane transporter of unknown function | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCAGTATCTTAGCTGAAAACCTTTGATA |
| Internal bar code: | CCCTTGCTAGCCTTTTTCATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 22 |
| LEAP-Seq percent confirming: | 99.7516 |
| LEAP-Seq n confirming: | 1606 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACGCGCCTAAAGAGTATC |
| Suggested primer 2: | CTTGCAAGAAAGAACCGAGG |