| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.186960 |
| Chromosome: | chromosome 12 |
| Location: | 244279 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485300 | CSV6 | Chlamydomonas-specific family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCGCAGTTCCAGGCAAGGCACCATTCGC |
| Internal bar code: | ACTGTAATCTTCGAGGGTCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 88 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 200 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCTTGTCGGTGTGGAGG |
| Suggested primer 2: | CTCAACAGCTCACTTGCTGC |