Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.186964 |
Chromosome: | chromosome 12 |
Location: | 3610945 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g513900 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTATGCAACAGGGCGAGGAGGAGAGCGTG |
Internal bar code: | AGCACTTCTGTTGGAGCGCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 908 |
LEAP-Seq percent confirming: | 99.0157 |
LEAP-Seq n confirming: | 4024 |
LEAP-Seq n nonconfirming: | 40 |
LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTCTTTGGCCCTCTCTCTT |
Suggested primer 2: | ATGTGTGCGCATTTGGTTTA |