| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186973 |
| Chromosome: | chromosome 6 |
| Location: | 6241067 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g290850 | (1 of 19) IPR018392 - LysM domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGAGCCGACATTGCTGCTTCCGGCATG |
| Internal bar code: | CTAGGGTGTCGGCCGCTCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 871 |
| LEAP-Seq percent confirming: | 33.288 |
| LEAP-Seq n confirming: | 3427 |
| LEAP-Seq n nonconfirming: | 6868 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTCAAGGCAATCTGTCA |
| Suggested primer 2: | ACAAACATGCACCCGATGTA |