Insertion junction: LMJ.RY0402.186980_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.186980
Chromosome:chromosome 4
Location:1228142
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g215250 FAP26 Ankyrin Repeat Flagellar Associated Protein 26; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAATCCAATCCCAAAATATATAAAACCAT
Internal bar code:CCGAATCGGGGGTGAAAGGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:963
LEAP-Seq percent confirming:93.5849
LEAP-Seq n confirming:248
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTTTCGTTTAGTTTGGGC
Suggested primer 2:GTTTCTTCCTCGTGCTCGTC