| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.186980 |
| Chromosome: | chromosome_4 |
| Location: | 1228142 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g215250 | FAP26 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCAATCCAATCCCAAAATATATAAAACCAT |
| Internal bar code: | CCGAATCGGGGGTGAAAGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 963 |
| LEAP-Seq percent confirming: | 93.5849 |
| LEAP-Seq n confirming: | 248 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTTCGTTTAGTTTGGGC |
| Suggested primer 2: | GTTTCTTCCTCGTGCTCGTC |