| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.186981 |
| Chromosome: | chromosome 2 |
| Location: | 6538653 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g116800 | (1 of 1) PTHR12381:SF46 - SAP DNA-BINDING DOMAIN-CONTAINING PROTEIN | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATTCTAGCTACGTTTACAATGTCTGCCT |
| Internal bar code: | AGGCGCTGACGTCGCTCCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 98.5554 |
| LEAP-Seq n confirming: | 614 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTGAGGTGGTGGATGAG |
| Suggested primer 2: | ACTCGGTCACCAGGACATTC |