Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.186984 |
Chromosome: | chromosome_1 |
Location: | 6928640 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g049950 | VLE1,SPO | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTTAAACACCAGTGCGCGACTGTCAAAAAC |
Internal bar code: | GGTTTTAATCAGACACCGGGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 560 |
LEAP-Seq percent confirming: | 99.6365 |
LEAP-Seq n confirming: | 3015 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTGCACCCGTGATGTGTAT |
Suggested primer 2: | CGGTGCCTCCTTAACATCAT |