Insertion junction: LMJ.RY0402.186990_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.186990
Chromosome:chromosome 6
Location:6822794
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g295100 EEP4 (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos); Endonuclease/exonuclease/phosphatase family protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGAAGCAGGCTGAGGTGGTTGCGGGCGC
Internal bar code:GTGTCCGGTACGCAACGGCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:327
LEAP-Seq percent confirming:99.7479
LEAP-Seq n confirming:1187
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTGTGTGTGTGTGTGTGT
Suggested primer 2:CGCGATTGTTTGTTTTGCTA