| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.186990 |
| Chromosome: | chromosome 6 |
| Location: | 6822884 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g295100 | EEP4 | (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos); Endonuclease/exonuclease/phosphatase family protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGATGCAGGCGTTGGGCTCAAGGCGTG |
| Internal bar code: | AGCACCCCCAGAGTGGAGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 758 |
| LEAP-Seq percent confirming: | 98.2734 |
| LEAP-Seq n confirming: | 6944 |
| LEAP-Seq n nonconfirming: | 122 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCCCTCGAAGTTGTAGC |
| Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |