| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.187002 |
| Chromosome: | chromosome 6 |
| Location: | 1501041 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259700 | (1 of 6) 3.1.2.2 - Palmitoyl-CoA hydrolase / Long-chain fatty-acyl-CoA hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCGGCCGTGCATGTGTGATGCGTCCGA |
| Internal bar code: | GGTCAATTGTAATGGAGCTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 102 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 217 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGTGCACTTCTACACCA |
| Suggested primer 2: | CAGGGTGGAGTAGGGAACAA |