| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.187002 |
| Chromosome: | chromosome 7 |
| Location: | 1961966 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325752 | FAP258 | Flagellar Associated Protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCCGCTGCCGCCGCCAGTGCAGCAGGG |
| Internal bar code: | ACAGCTCCAAGAATCCCCGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 190 |
| LEAP-Seq percent confirming: | 97.028 |
| LEAP-Seq n confirming: | 555 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATAGAGCAGCAAGGGTGC |
| Suggested primer 2: | CTGTGTGAGGCAGTAGCAGC |