Insertion junction: LMJ.RY0402.187011_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.187011
Chromosome:chromosome 10
Location:2396135
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g435350 ESU1,ESU1a Endosulfine cAMP-regulated phosphoprotein; (1 of 2) PF04667 - cAMP-regulated phosphoprotein/endosulfine conserved region (Endosulfine) 5'UTR/5'UTR_intron|CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCAGAGAGGCGCAAGGAGTTGTCGCAAG
Internal bar code:TCCGCGCGAGATGGCGTAGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:976
LEAP-Seq percent confirming:99.6785
LEAP-Seq n confirming:1550
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGCTATCAGTGCGTCCAG
Suggested primer 2:CGAGCCAAGAAGTTGAGACC