| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.187015 |
| Chromosome: | chromosome 1 |
| Location: | 2715416 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016100 | (1 of 7) IPR011048 - Cytochrome cd1-nitrite reductase-like, haem d1 domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTATCGGCCCCAGCATCCTGCATAACAGC |
| Internal bar code: | AGCGCTTTACGTCTCGTCGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 99.8651 |
| LEAP-Seq n confirming: | 2221 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGTCAACGCAAGGCTGT |
| Suggested primer 2: | TTGCAGTGTGCAATGCTGTA |