| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.187015 |
| Chromosome: | chromosome 7 |
| Location: | 5698632 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352600 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGGTGAGGCGCCGGACCGCATGAGGACA |
| Internal bar code: | AGGGCCAGTGGCGCACTAGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 349 |
| LEAP-Seq percent confirming: | 93.8036 |
| LEAP-Seq n confirming: | 2407 |
| LEAP-Seq n nonconfirming: | 159 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATTTATTCTGGGCGTTGC |
| Suggested primer 2: | CCTCCCACAGCTCAAAGAAG |