| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187031 |
| Chromosome: | chromosome 2 |
| Location: | 7437264 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGAACATTCATGCCACGCCTCAGCATGG |
| Internal bar code: | TTCTTGACGTGCCGCGTCATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1030 |
| LEAP-Seq percent confirming: | 95.6316 |
| LEAP-Seq n confirming: | 810 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGATCTAGTAGCCAGCCG |
| Suggested primer 2: | ATAGCAGGTTCGGTACGGTG |