Insertion junction: LMJ.RY0402.187038_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.187038
Chromosome:chromosome 1
Location:2054520
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g010950 (1 of 2) PF00023//PF12796//PF13920 - Ankyrin repeat (Ank) // Ankyrin repeats (3 copies) (Ank_2) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGACGAAAGCAGACGGCTGCGTGTGTGGG
Internal bar code:GCCCCACTTACGTTGGGCTTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:977
LEAP-Seq percent confirming:99.8748
LEAP-Seq n confirming:798
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACACCGGGTTATCGAAGATG
Suggested primer 2:GTTCAGAAAAGGGCTAGGGG