Insertion junction: LMJ.RY0402.187050_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.187050
Chromosome:chromosome 10
Location:15006
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g417500 CYN20E,CYN20-5,CYN9 (1 of 1) PF00160//PF04969 - Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD (Pro_isomerase) // CS domain (CS); Cyclophilin 20-5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTATCTGCAGGAACAAACGGGAGCCAGTTC
Internal bar code:ATGGGTCCTCGCGAATCACCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:110
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGAAATCTGCTGTGCCACTA
Suggested primer 2:CACATATGGTTGCCCCCTAC