Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.187075 |
Chromosome: | chromosome_11 |
Location: | 331254 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g467567 | UBQ1 | Ubiquitin | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCCCGACTGTATCAACAAGAAGCACCGCAC |
Internal bar code: | CCACAGTAGTAATCGTCTCTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 164 |
LEAP-Seq percent confirming: | 99.5249 |
LEAP-Seq n confirming: | 419 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCATTAAGGCGGTGAGAAAA |
Suggested primer 2: | CTCGGATTCCGTGATCTTGT |