| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187075 |
| Chromosome: | chromosome_11 |
| Location: | 331269 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g467567 | UBQ1 | Ubiquitin | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTACGCTTGAACGACGTGTAAATTTGTATG |
| Internal bar code: | GTTGACTTTTCGGCCGCAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 96.2413 |
| LEAP-Seq n confirming: | 1101 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATTAAGGCGGTGAGAAAA |
| Suggested primer 2: | CTCGGATTCCGTGATCTTGT |