| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187084 |
| Chromosome: | chromosome 7 |
| Location: | 827565 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318426 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGCGCCGTGCAGGGAGGAAGCTGCTGG |
| Internal bar code: | CCGGACTAGGGATATGGGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 980 |
| LEAP-Seq percent confirming: | 99.6415 |
| LEAP-Seq n confirming: | 7782 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTTCTACCTCGCACTCC |
| Suggested primer 2: | GAACACTTCCTCGACTTCGC |