| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.187090 |
| Chromosome: | chromosome_2 |
| Location: | 2809100 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g094450 | PHC21 | Cell wall protein pherophorin-C21 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACTAGCAGCTTTGACTCTCAACACGGCCT |
| Internal bar code: | GCAAGGGTTCAGGCTTTCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 98.3793 |
| LEAP-Seq n confirming: | 5281 |
| LEAP-Seq n nonconfirming: | 87 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTAAGGCGGGTAGCAAT |
| Suggested primer 2: | AAGGGCTATGTGTACAGCCG |