| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187103 |
| Chromosome: | chromosome 14 |
| Location: | 943749 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613850 | (1 of 1) K12585 - exosome complex exonuclease DIS3/RRP44 (DIS3, RRP44) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACACTGCCGCGACCCGCCATCTGGAGAG |
| Internal bar code: | GGCGGTATCACGGCCACGTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 360 |
| LEAP-Seq percent confirming: | 61.2639 |
| LEAP-Seq n confirming: | 1047 |
| LEAP-Seq n nonconfirming: | 662 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCCGCTACCTACCTACC |
| Suggested primer 2: | GGAGTACTGAAACCCCACGA |