Insertion junction: LMJ.RY0402.187111_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.187111
Chromosome:chromosome 6
Location:3004920
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g273650 (1 of 3) 1.11.2.3 - Plant seed peroxygenase / Soybean peroxygenase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTCGGCAAAAGCCACACCTGGATGCCCC
Internal bar code:GTACGAGAGGTTTCCTCGCGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:933
LEAP-Seq percent confirming:99.6964
LEAP-Seq n confirming:2299
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GCCATCCACCTCAAGAACAT
Suggested primer 2:GACGTGGGGTCTTCTTGTGT