Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.187142 |
Chromosome: | chromosome_10 |
Location: | 940499 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g424600 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGGGCTGCCGCTGGCGCTAGAGGATTGGG |
Internal bar code: | AGTGGGCCCTCCGTCCATGGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 316 |
LEAP-Seq percent confirming: | 99.3727 |
LEAP-Seq n confirming: | 3010 |
LEAP-Seq n nonconfirming: | 19 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAACCATTAGCACCCGAGAT |
Suggested primer 2: | GTGCTGGCAGTTAGGCTAGG |