Insertion junction: LMJ.RY0402.187146_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.187146
Chromosome:chromosome 16
Location:6576858
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g672200 BALD11,BLD11 (1 of 1) PF14309 - Domain of unknown function (DUF4378) (DUF4378); Bald 11 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCGCGCACGCTGTTGAGAAGTCCAGTTT
Internal bar code:TCTCCTCGGTGACCAACTGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:712
LEAP-Seq percent confirming:99.2908
LEAP-Seq n confirming:840
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTTCATAAGTGCTGTGGA
Suggested primer 2:AAAGGACTCATCGCTGCTGT