| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187157 |
| Chromosome: | chromosome 16 |
| Location: | 5381360 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681500 | (1 of 1) K07002 - uncharacterized protein (K07002) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGGTACGGCAGAAGGGGGCAAGGTGGTG |
| Internal bar code: | AGGTTGATCAGGTTCCGGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 96.4775 |
| LEAP-Seq n confirming: | 45356 |
| LEAP-Seq n nonconfirming: | 1656 |
| LEAP-Seq n unique pos: | 136 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACGTACGGTAGTGAGGT |
| Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |