| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.187165 |
| Chromosome: | chromosome 2 |
| Location: | 7327333 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g145200 | (1 of 1) 4.1.1.9 - Malonyl-CoA decarboxylase / Malonyl-CoA carboxy-lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCACCGGCGCGGTCGTGGTGCGACAGGA |
| Internal bar code: | TGCTGCTCCAGACCGGTGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 326 |
| LEAP-Seq percent confirming: | 81.2977 |
| LEAP-Seq n confirming: | 852 |
| LEAP-Seq n nonconfirming: | 196 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTAAGGAGGCGAGAAGGC |
| Suggested primer 2: | AGGCAGAGGCGTTAGTTGAA |