| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187197 |
| Chromosome: | chromosome 17 |
| Location: | 181635 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697450 | (1 of 1) K15029 - translation initiation factor 3 subunit L (EIF3L) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAACATTGCATTGCGCCAGTGAGCGCTG |
| Internal bar code: | GGGGGCGGGGATACGCGAGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 380 |
| LEAP-Seq percent confirming: | 99.4692 |
| LEAP-Seq n confirming: | 937 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAAACTCGCTTACCCGCAG |
| Suggested primer 2: | CAGCCGTCGTACAATCACAC |