Insertion junction: LMJ.RY0402.187220_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.187220
Chromosome:chromosome 7
Location:2075341
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g326010 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTGCCTCTACCGATGCGTTCATACTTCA
Internal bar code:TTGTAAACCTGACCTGGCGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:461
LEAP-Seq percent confirming:99.6049
LEAP-Seq n confirming:2269
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACAATCCGACTCACC
Suggested primer 2:AAAACTACTCGCCAACGCAT