Insertion junction: LMJ.RY0402.187235_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.187235
Chromosome:chromosome_2
Location:3650880
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre02.g095149 FAP143 Flagellar Associated Protein sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TCTAATGAGAAGGCGCGTGGTAGCGGTAGG
Internal bar code:GAATGAGAAGGCCGAGGCGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:961
LEAP-Seq percent confirming:99.1941
LEAP-Seq n confirming:4677
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:TGAAGGAAGCAGCATTTGTG
Suggested primer 2:CAGTGTTGCCAATTACACGG