| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187264 |
| Chromosome: | chromosome 12 |
| Location: | 7462605 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556250 | SEP1 | Septin-like protein; (1 of 1) K16944 - septin 7 (SEPT7, CDC3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACCTGGATGCGTGCTTTTGTTGGAAGT |
| Internal bar code: | ATGGTGTAGATAGGGCAGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 526 |
| LEAP-Seq percent confirming: | 98.8359 |
| LEAP-Seq n confirming: | 5264 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTACCGCTTTTCAGGTGG |
| Suggested primer 2: | GAGTGCTCCTTGTTGAAGGC |