| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.187265 |
| Chromosome: | chromosome 12 |
| Location: | 4442290 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521300 | POB14 | Proteome of basal body 14 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCCTGGAAAGTGTTTCGGGTCCACGTC |
| Internal bar code: | GTATGGCTATCGCCCTGCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 99.4652 |
| LEAP-Seq n confirming: | 3906 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCATGTTGGTTGATGCGT |
| Suggested primer 2: | GCGCGTGACACTGTGTTAGT |