| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.187266 |
| Chromosome: | chromosome 10 |
| Location: | 1267228 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426900 | (1 of 3) PF02458 - Transferase family (Transferase) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACGTGGTGGTACGGTTGTTGACGCGTGG |
| Internal bar code: | TCGTTAAATCTGTGTCGGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 99.6305 |
| LEAP-Seq n confirming: | 3505 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGTCTTAGAAGCGGTTG |
| Suggested primer 2: | GCTGCGTGTTGCTGTAGTGT |