| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.187302 |
| Chromosome: | chromosome 14 |
| Location: | 3944842 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633100 | (1 of 1) PF12070 - Protein of unknown function (DUF3550/UPF0682) (DUF3550) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTTCCGGGTTGCTGTCGTGTGTGAGCCG |
| Internal bar code: | AATCACGTGAAGTACCACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 597 |
| LEAP-Seq percent confirming: | 99.5788 |
| LEAP-Seq n confirming: | 2128 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGAGAAACCTGACACGGG |
| Suggested primer 2: | ACACTAGCGCACACGACAAC |