| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.187328 |
| Chromosome: | chromosome 8 |
| Location: | 2134770 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g369100 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCTGAATGCGCATGGCCCAGGCATTTG |
| Internal bar code: | GCACGGTGGCATTTGCCTCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 99.5468 |
| LEAP-Seq n confirming: | 659 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGACACAAGCGAGTGGTG |
| Suggested primer 2: | CGCACGGAATGAAGTGACTA |