| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.187340 |
| Chromosome: | chromosome 11 |
| Location: | 660428 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467620 | CEP83 | Centrosomal protein 83 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGTCACGGCATGGACGATCCACGCGAAT |
| Internal bar code: | TAAGGCATATCCGCGGAAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 516 |
| LEAP-Seq percent confirming: | 96.3203 |
| LEAP-Seq n confirming: | 3717 |
| LEAP-Seq n nonconfirming: | 142 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCGTTCTTGACCTCATCA |
| Suggested primer 2: | CTGTGGCTCACTCCTGTTGA |