Insertion junction: LMJ.RY0402.187340_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.187340
Chromosome:chromosome 11
Location:660492
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467620 CEP83 Centrosomal protein 83 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCTTGTTCCAGGCCCTGGCTCTCCCCTC
Internal bar code:AGAACTCAGAATAATCACGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:392
LEAP-Seq percent confirming:49.3711
LEAP-Seq n confirming:1413
LEAP-Seq n nonconfirming:1449
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGGCTCACTCCTGTTGA
Suggested primer 2:GTTCTCCACATGAACGGCTT