| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187341 |
| Chromosome: | chromosome 5 |
| Location: | 784668 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248300 | NRAMP4 | Manganese/metal transporter, NRAMP homolog; (1 of 1) K12347 - natural resistance-associated macrophage protein (SLC11A, NRAMP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAAGAGGAGTGTGGGGTTTCGCTAACC |
| Internal bar code: | GAGGAAGTGTATAGCGTGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 454 |
| LEAP-Seq percent confirming: | 99.7906 |
| LEAP-Seq n confirming: | 4288 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACACCCAACAACTACGCC |
| Suggested primer 2: | ACGCGCACACACTCTCATAC |