| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.187350 |
| Chromosome: | chromosome 11 |
| Location: | 749371 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467631 | CGL90 | Zinc carboxypeptidase; Conserved in green lineage; (1 of 1) 3.4.17.15 - Carboxypeptidase A2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGTAGTCGGGCTGTGCCCATAATAGCC |
| Internal bar code: | CTTTTCCAACGCGTGACGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 217 |
| LEAP-Seq percent confirming: | 90.6158 |
| LEAP-Seq n confirming: | 618 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGTATCTTCACACACACG |
| Suggested primer 2: | GCGGTTCTCGAGTAGACGAC |