Insertion junction: LMJ.RY0402.187362_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.187362
Chromosome:chromosome 2
Location:6439071
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g115850 (1 of 3) PF01987 - Mitochondrial biogenesis AIM24 (AIM24) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCACTCATCAACGCGTGGCTGTAGCCACT
Internal bar code:TGCCCATATCGGCGCTCATCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:556
LEAP-Seq percent confirming:78.866
LEAP-Seq n confirming:459
LEAP-Seq n nonconfirming:123
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTTGGCTAGGACTCACTG
Suggested primer 2:ATGTTTTCTCGGAAACACGG