Insertion junction: LMJ.RY0402.187425_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.187425
Chromosome:chromosome 7
Location:2203731
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g327100 (1 of 12) IPR000326 - Phosphatidic acid phosphatase type 2/haloperoxidase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGCAAAGAGGAGACTAGGAGAGCTACGA
Internal bar code:GCCTATCATGCATACAACTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:761
LEAP-Seq percent confirming:99.7271
LEAP-Seq n confirming:1462
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTGGTTTGGTTTCGTTCG
Suggested primer 2:CTCAACGTCGAGCAGGTACA