| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187426 |
| Chromosome: | chromosome 5 |
| Location: | 2607007 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236250 | Conserved WD40-repeat Protein; (1 of 2) PTHR22847//PTHR22847:SF459 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGTGACAAGACATTCTTTGGGCGGCT |
| Internal bar code: | TCCGCGACACCCCAATGACTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 63 |
| LEAP-Seq percent confirming: | 84.7352 |
| LEAP-Seq n confirming: | 272 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAATCTGCGCTTTGTGTC |
| Suggested primer 2: | TCGAATTCATCCTTATCGCC |