| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.187469 |
| Chromosome: | chromosome 11 |
| Location: | 509182 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467598 | (1 of 1) IPR000104//IPR013763 - Antifreeze protein, type I // Cyclin-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTGGGCGGGCAGGGGTGCCGGCCTGTG |
| Internal bar code: | TACGCCTCCTGAGGGCTATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 588 |
| LEAP-Seq percent confirming: | 98.3623 |
| LEAP-Seq n confirming: | 961 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTTGTAGAGCTTTGCCGC |
| Suggested primer 2: | GAGCGATACCCATTCATGCT |