| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187478 |
| Chromosome: | chromosome_9 |
| Location: | 6539370 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g407801 | AKC1 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAACATTGTGCCATTGACATCATGCACAAC |
| Internal bar code: | AGGCGGGAATTCACCTATCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 485 |
| LEAP-Seq percent confirming: | 97.0528 |
| LEAP-Seq n confirming: | 955 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAACAGATCAACAGCAGC |
| Suggested primer 2: | CGGTTTAGTTTGGGCTGGTA |