| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.187493 |
| Chromosome: | chromosome 10 |
| Location: | 465202 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420950 | (1 of 45) IPR029052 - Metallo-dependent phosphatase-like | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTTAGTTGGGGCAAAAGGCGAACTGAAG |
| Internal bar code: | AAAAACGGTCCGGCACGTCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 571 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 215 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCAAAAGCTTGGCATGAG |
| Suggested primer 2: | AAGTGCTGGGAGTAACGGTG |