| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.187513 |
| Chromosome: | chromosome 3 |
| Location: | 3549703 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g167924 | (1 of 2) 4.2.1.134 - Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATCCGCCCATGCCATCCATCCCATTCA |
| Internal bar code: | CCGTTGACCGAGATGCAGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 85 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCAAGTGTGGTATCGGGC |
| Suggested primer 2: | CTGAGCCACACAACTCTGGA |